The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Murine MIP-1alpha by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1alpha. To detect Murine MIP-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine MIP-1alpha (
LS-C104628) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1alpha. Western Blot: To detect Murine MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine MIP-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.