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Catalog Number Size Price
LS-C154132-100 100 µg (1 mg/ml) $567 
CCL20 / MIP-3-Alpha Antibody - Anti-Mouse MIP3a Antibody - Western Blot. Western blot of anti-Mouse MIP3a antibody shows detection of a band ~11 kD in size corresponding to recombinant mouse MIP3a. Recombinant mouse MIP-3a was loaded on to an SDS-PAGE gel at 0.25 ug and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (MB-070) for 30 min at RT followed by reaction with FemtoMax Chemiluminescent substrate (p/n FEMTOMAX-110). Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.

Polyclonal Rabbit anti‑Mouse CCL20 / MIP‑3‑Alpha Antibody (WB) LS‑C154132

Polyclonal Rabbit anti‑Mouse CCL20 / MIP‑3‑Alpha Antibody (WB) LS‑C154132

Antibody:
CCL20 / MIP-3-Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C154132-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CCL20 / MIP-3-Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MIP-3-Alpha antibody LS-C154132 is an unconjugated rabbit polyclonal antibody to MIP-3-Alpha (CCL20) from mouse. It is reactive with mouse and rat. Validated for ELISA and WB.
Target
Mouse CCL20 / MIP-3-Alpha
Synonyms
CCL20 | Beta chemokine exodus-1 | Beta-chemokine exodus-1 | CKb4 | Exodus-1 | MIP-3a | MIP-3-alpha | LARC | ST38 | SCYA20 | C-C motif chemokine 20 | CC chemokine LARC | Mip-3alpha | MIP3A | Small-inducible cytokine A20
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3a protein.
Specificity
This antibody will recognize both native and recombinant mouse MIP-3a in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Applications
  • Western blot (1:1000)
  • ELISA (1:10000)
Usage
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 10.7 kD in size corresponding to native mouse MIP-3a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL20 / MIP-3-Alpha
P78556 NM_004591 NP_004582.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024