Target
Human CCL11 / Eotaxin
Synonyms
CCL11 | C-C motif chemokine 11 | Eotaxin-1 | SCYA11 | Small-inducible cytokine A11 | Eosinophil chemotactic protein | Eotaxin
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QDSMKYLDQKSPTPK, from the C-Terminus of protein sequence according to NP_002977.1.
Specificity
Human CCL11 / Eotaxin.
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western Blot: No specific signals and low background in Human Colon, Duodenum, Ileum and Lung lysates - our routinely used western blotting protocol does not allow detection of proteins as small as the ligand derived from its precursor or the precursor itself with calculated size of 10.7kD according to NP_002977.1. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any assay results been reported with other antibodies?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee