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Catalog Number Size Price
LS-C344024-10 10 µg $318 
LS-C344024-100 100 µg (0.5 mg/ml) $470 
CAV1 / Caveolin 1 Antibody - IHC analysis of Caveolin-1 using anti-Caveolin-1 antibody. Caveolin-1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Caveolin-1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CAV1 / Caveolin 1 Antibody - Caveolin-1 antibody IHC-paraffin: Human Lung Cancer Tissue.
CAV1 / Caveolin 1 Antibody - Caveolin-1 antibody IHC-paraffin: Human Placenta Tissue.
CAV1 / Caveolin 1 Antibody - IF analysis of Caveolin-1 using anti-Caveolin-1 antibody. Caveolin-1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Caveolin-1 Antibody overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:200 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CAV1 / Caveolin 1 Antibody - Caveolin-1 antibody Western blot. All lanes: Anti Caveolin-1 at 0.5 ug/ml. Lane 1: HeLa Whole Cell Lysate at 40 ug. Lane 2: HT1080 Whole Cell Lysate at 40 ug. Lane 3: Human Placenta Tissue Lysate at 50 ug. Lane 4: A431 Whole Cell Lysate at 40 ug. Predicted band size: 21 kD. Observed band size: 21 kD.
CAV1 / Caveolin 1 Antibody - Caveolin-1 antibody Western blot. All lanes: Anti Caveolin-1 at 0.5 ug/ml. WB: Recombinant Human Caveolin-1 Protein 0.5ng. Predicted band size: 40 kD. Observed band size: 40 kD.
CAV1 / Caveolin 1 Antibody - Flow Cytometry analysis of HeLa cells using anti-Caveolin-1 antibody. Overlay histogram showing HeLa cells stained with anti-Caveolin-1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CAV1 / Caveolin 1 Antibody - Flow Cytometry analysis of A549 cells using anti-Caveolin-1 antibody. Overlay histogram showing A549 cells stained with anti-Caveolin-1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CAV1 / Caveolin 1 Antibody - IHC analysis of Caveolin-1 using anti-Caveolin-1 antibody. Caveolin-1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Caveolin-1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CAV1 / Caveolin 1 Antibody - Caveolin-1 antibody IHC-paraffin: Human Lung Cancer Tissue.
CAV1 / Caveolin 1 Antibody - Caveolin-1 antibody IHC-paraffin: Human Placenta Tissue.
CAV1 / Caveolin 1 Antibody - IF analysis of Caveolin-1 using anti-Caveolin-1 antibody. Caveolin-1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Caveolin-1 Antibody overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:200 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CAV1 / Caveolin 1 Antibody - Caveolin-1 antibody Western blot. All lanes: Anti Caveolin-1 at 0.5 ug/ml. Lane 1: HeLa Whole Cell Lysate at 40 ug. Lane 2: HT1080 Whole Cell Lysate at 40 ug. Lane 3: Human Placenta Tissue Lysate at 50 ug. Lane 4: A431 Whole Cell Lysate at 40 ug. Predicted band size: 21 kD. Observed band size: 21 kD.
CAV1 / Caveolin 1 Antibody - Caveolin-1 antibody Western blot. All lanes: Anti Caveolin-1 at 0.5 ug/ml. WB: Recombinant Human Caveolin-1 Protein 0.5ng. Predicted band size: 40 kD. Observed band size: 40 kD.
CAV1 / Caveolin 1 Antibody - Flow Cytometry analysis of HeLa cells using anti-Caveolin-1 antibody. Overlay histogram showing HeLa cells stained with anti-Caveolin-1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CAV1 / Caveolin 1 Antibody - Flow Cytometry analysis of A549 cells using anti-Caveolin-1 antibody. Overlay histogram showing A549 cells stained with anti-Caveolin-1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CAV1 / Caveolin 1 Antibody (aa4‑178, IHC, WB) LS‑C344024

Polyclonal Rabbit anti‑Human CAV1 / Caveolin 1 Antibody (aa4‑178, IHC, WB) LS‑C344024

Note: This antibody replaces LS-C388146
Antibody:
CAV1 / Caveolin 1 Rabbit anti-Human Polyclonal (aa4-178) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344024-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CAV1 / Caveolin 1 Rabbit anti-Human Polyclonal (aa4-178) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Caveolin 1 antibody LS-C344024 is an unconjugated rabbit polyclonal antibody to Caveolin 1 (CAV1) (aa4-178) from human. It is reactive with human and mouse. Validated for IHC and WB.
Target
Human CAV1 / Caveolin 1
Synonyms
CAV1 | Caveolin-1 | CGL3 | CAV | MSTP085 | VIP21 | BSCL3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Caveolin-1, respectively.
Epitope
aa4-178
Specificity
Expressed in muscle and lung, less so in liver, brain and kidney.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CAV1 / Caveolin 1
Q03135 NM_001753 NP_001744.2

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Requested From: United States
Date Requested: 11/22/2024