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Catalog Number	Size	Price
LS-C756625-10	10 µg	$318
LS-C756625-100	100 µg	$470

CASR/Calcium Sensing Receptor Antibody - Western blot analysis of CASR using anti-CASR antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human A549 whole cell lysate,Lane 3: human 22RV1 whole cell lysate,Lane 4: human HepG2 whole cell lysate,Lane 5: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CASR antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

CASR/Calcium Sensing Receptor Antibody - Flow Cytometry analysis of NEURO-2A cells using anti-CASR antibody. Overlay histogram showing NEURO-2A cells stained with anti-CASR antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CASR Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Monoclonal Mouse anti‑Human CASR/Calcium Sensing Receptor Antibody (IHC, WB) LS‑C756625

Name: Anti-CASR/Calcium Sensing Receptor Antibody | Mouse anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C756625-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CASR/Calcium Sensing Receptor Mouse anti-Human Monoclonal Antibody

Application:

IHC-P, IHC-Fr, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C756625-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CASR/Calcium Sensing Receptor Mouse anti-Human Monoclonal Antibody

Application:

IHC-P, IHC-Fr, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Calcium Sensing Receptor antibody LS-C756625 is an unconjugated mouse monoclonal antibody to human Calcium Sensing Receptor (CASR). Validated for Flow, ICC, IHC and WB.

Target

Human CASR/Calcium Sensing Receptor

Synonyms

CASR | Ca sensing receptor | Ca2+ sensing receptor | Calcium-sensing receptor | FIH | EIG8 | GPRC2A | HHC | HHC1 | Hypocalciuric hypercalcemia 1 | PCAR1 | NSHPT | CAR | FHH

See All CASR/Calcium Sensing Receptor Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG2b Monoclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived human CASR recombinant protein (Position: Q926-S1078). Human CASR shares 80.5% and 78.6% amino acid (aa) sequence identity with mouse and rat CASR, respectively.

Specificity

No cross reactivity with other proteins.

Applications

IHC - Paraffin (0.5 - 1 µg/ml)
IHC - Frozen (0.5 - 1 µg/ml)
ICC (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
Flow Cytometry (1 - 3 µg/10E6 cells)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.