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Antibodies > CASP8 / Caspase 8 > LS-C344799
 
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Catalog Number Size Price
LS-C344799-100 100 µg $414 
CASP8 / Caspase 8 Antibody - Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p20 subunit. Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue.
CASP8 / Caspase 8 Antibody - Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.
CASP8 / Caspase 8 Antibody - Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.
CASP8 / Caspase 8 Antibody - Flow cytometry data in permeabilized cells of overexpressed FLAG-tagged pro-caspase-8 (active-site-mutant) in 293T cells using anti-caspase-8 mAbs (3B10) , anti-FLAG or control. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed.
CASP8 / Caspase 8 Antibody - Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p20 subunit. Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue.
CASP8 / Caspase 8 Antibody - Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.
CASP8 / Caspase 8 Antibody - Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.
CASP8 / Caspase 8 Antibody - Flow cytometry data in permeabilized cells of overexpressed FLAG-tagged pro-caspase-8 (active-site-mutant) in 293T cells using anti-caspase-8 mAbs (3B10) , anti-FLAG or control. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed.
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Monoclonal Rat anti‑Mouse CASP8 / Caspase 8 Antibody (clone 3B10, IHC, WB) LS‑C344799

Monoclonal Rat anti‑Mouse CASP8 / Caspase 8 Antibody (clone 3B10, IHC, WB) LS‑C344799

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Antibody:
CASP8 / Caspase 8 Rat anti-Mouse Monoclonal (3B10) Antibody
Application:
IHC, ICC, WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$414
LS-C344799-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CASP8 / Caspase 8 Rat anti-Mouse Monoclonal (3B10) Antibody
Application:
IHC, ICC, WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 8 antibody LS-C344799 is an unconjugated rat monoclonal antibody to mouse Caspase 8 (CASP8). Validated for ELISA, Flow, ICC, IHC and WB.
Target
Mouse CASP8 / Caspase 8
Synonyms
CASP8 | ALPS2B | Apoptotic protease Mch-5 | Apoptotic cysteine protease | Casp-8 | Caspase-8 | CAP4 | Caspase 8 | MACH | MCH5 | MORT1-associated ced-3 homolog | ICE-like apoptotic protease 5 | Pro Caspase 8 | FADD-like ICE | FLICE | MACH-alpha-1/2/3 protein | MACH-beta-1/2/3/4 protein
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
3B10
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Recombinant mouse caspase-8 (p20 subunit).
Specificity
Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.
Applications
  • IHC
  • ICC
  • Western blot (1 - 2 µg/ml)
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
ELISA - Recombinant protein. Flow Cytometry - Overexpressed in cell lines. Immunocytochemistry - Excellent, see literature reference 2. Western Blot - 1-2 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP8 / Caspase 8
Q14790 nm_001228 AAC50645.1

Publications (0)

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CASP8 / Caspase 8 Antibody
CASP8 / Caspase 8 Antibody (clone 5F7) LS‑C179764
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CASP8 / Caspase 8 Antibody - Immunofluorescence analysis of U2OS cells using CASP8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CASP8 / Caspase 8 Antibody LS‑C746976
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CASP8 / Caspase 8 Antibody - Western blot analysis of lysates from LOVO cells treated with UV 15', using Caspase 8 (Phospho-Tyr380) Antibody. The lane on the right is blocked with the phospho peptide.
CASP8 / Caspase 8 Antibody (phospho‑Tyr380) LS‑C198731
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all CASP8 / Caspase 8 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
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