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Catalog Number Size Price
LS-C662763-10 10 µg $318 
LS-C662763-100 100 µg $470 
CASP4 / Caspase 4 Antibody - Immunohistochemistry - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - Immunohistochemistry - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - IF analysis of Caspase 4 using anti-Caspase 4 antibody. Caspase 4 was detected in paraffin-embedded section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Caspase 4 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CASP4 / Caspase 4 Antibody - Western blot - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - Flow Cytometry analysis of A431 cells using anti-Caspase 4 antibody. Overlay histogram showing A431 cells stained with anti-Caspase 4 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 4 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CASP4 / Caspase 4 Antibody - Immunohistochemistry - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - Immunohistochemistry - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - IF analysis of Caspase 4 using anti-Caspase 4 antibody. Caspase 4 was detected in paraffin-embedded section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Caspase 4 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CASP4 / Caspase 4 Antibody - Western blot - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - Flow Cytometry analysis of A431 cells using anti-Caspase 4 antibody. Overlay histogram showing A431 cells stained with anti-Caspase 4 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 4 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CASP4 / Caspase 4 Antibody (IHC, WB) LS‑C662763

Polyclonal Rabbit anti‑Human CASP4 / Caspase 4 Antibody (IHC, WB) LS‑C662763

Antibody:
CASP4 / Caspase 4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662763-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CASP4 / Caspase 4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 4 antibody LS-C662763 is an unconjugated rabbit polyclonal antibody to human Caspase 4 (CASP4). Validated for ELISA, IHC and WB.
Target
Human CASP4 / Caspase 4
Synonyms
CASP4 | CASP-4 | Casp11 | Caspase 4 | Caspase-4 | Cysteine protease icerel-ii | ICE(rel)-II | ICE(rel)II | ICH-2 | Ich-3 | ICH2 | Protease ICH-2 | TX | Tx protease | Caspase 11 | ICEREL-II | Mih1/TX | Protease TX
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human Caspase 4 recombinant protein (Position: H111-R194).
Specificity
Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP4 / Caspase 4
P49662 NM_001225 NP_001216.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/21/2024