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Catalog Number Size Price
LS-C814745-100 100 µl $412 
CAP1 Antibody - Immunofluorescence staining of CAP1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human CAP1 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
CAP1 Antibody - Anti-CAP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 52 kDa.
CAP1 Antibody - CAP1 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 4 uL anti-CAP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CAP1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 50 kDa.
CAP1 Antibody - Immunofluorescence staining of CAP1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human CAP1 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
CAP1 Antibody - Anti-CAP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 52 kDa.
CAP1 Antibody - CAP1 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 4 uL anti-CAP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CAP1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 50 kDa.
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Polyclonal Rabbit anti‑Human CAP1 Antibody (IF, WB) LS‑C814745

Polyclonal Rabbit anti‑Human CAP1 Antibody (IF, WB) LS‑C814745

Antibody:
CAP1 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$412
LS-C814745-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CAP1 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CAP1 antibody LS-C814745 is an unconjugated rabbit polyclonal antibody to human CAP1. Validated for ICC, IF, IP and WB.
Target
Human CAP1
Synonyms
CAP1 | CAP | CAP 1 | CAP1-PEN | SSKAP55
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to the center region of the Human CAP1
Specificity
Human CAP1
Applications
  • ICC (1:300 - 1:10000)
  • Immunofluorescence (1:300 - 1:10000)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CAP1
Q01518 NM_006367 NP_006358.1

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Requested From: United States
Date Requested: 11/26/2024