Human Calprotectin

Mouse

Human, Bovine, Rhesus monkey (tested or 100% immunogen sequence identity)

Porcine

Monoclonal

27E10

Unconjugated

Affinity purified

Unmodified

Cultured human monocytes

The antigen is MRP8/14 (calprotectin), the epitope involves parts of both subunits MRP8 and MRP14. Antigen distribution: Isolated cells: Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80% of monocytes in early cultures (24- 48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue sections: The antigen is found in macrophages in the red pulp of the spleen and liver. It is strongly expressed in macrophages from acute inflamed tissues (peridontitis, contact eczema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen. It is normally absent on resident mononuclear phagocytes in healthy tissues (skin, gut, thymus). Specificity: Human: subpopulation of macrophages, monocytes and granulocytes; peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Other: subpopulation of macrophages of rhesus monkey and bovine tissues. The antibody does not react with swine tissues.

• IHC
• IHC - Paraffin (1 µg/ml)
• ELISA

• IHC - Frozen

For IHC-P, Proteinase K pretreatment is recommended for antigen retrieval.

Lyophilized from PBS pH 7.2, 5mg/ml BSA, and 0.05% Kathon CG

Reconstitute with 0.5ml distilled H2O.

Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.