LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > CACNA2D2 > LS-C756493
 
Print Page  
Catalog Number Size Price
LS-C756493-10 10 µg $318 
LS-C756493-100 100 µg $470 
CACNA2D2 Antibody - Western blot analysis of CACNA2D2 using anti-CACNA2D2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA2D2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CACNA2D2 at approximately 130KD. The expected band size for CACNA2D2 is at 130KD.
CACNA2D2 Antibody - Flow Cytometry analysis of A549 cells using anti-CACNA2D2 antibody. Overlay histogram showing A549 cells stained with anti-CACNA2D2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CACNA2D2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CACNA2D2 Antibody - Western blot analysis of CACNA2D2 using anti-CACNA2D2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA2D2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CACNA2D2 at approximately 130KD. The expected band size for CACNA2D2 is at 130KD.
CACNA2D2 Antibody - Flow Cytometry analysis of A549 cells using anti-CACNA2D2 antibody. Overlay histogram showing A549 cells stained with anti-CACNA2D2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CACNA2D2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human CACNA2D2 Antibody (IHC, WB) LS‑C756493

Polyclonal Rabbit anti‑Human CACNA2D2 Antibody (IHC, WB) LS‑C756493

Submit Review LSBio Guarantee
Antibody:
CACNA2D2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-Fr, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756493-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CACNA2D2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-Fr, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CACNA2D2 antibody LS-C756493 is an unconjugated rabbit polyclonal antibody to CACNA2D2 from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
Target
Human CACNA2D2
Synonyms
CACNA2D2 | CACNA2D | Gene 26 | KIAA0558 | LUAC11.1 | Alpha-2-delta-B | Alpha2delta-2 | Ducky
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human CACNA2D2 recombinant protein (Position: Q66-Q284).
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Frozen (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Flow Cytometry (1 - 3 µg/10E6 cells)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CACNA2D2
Q9NY47 nm_006030 NP_006021.2

Publications (0)

Submit a Publication for LS-C756493

Customer Reviews (0)

Submit a review for LS-C756493

Featured Products

Product
CACNA2D2 Antibody (aa643‑671, Biotin) LS‑C253285
Species: Mouse, Human
Applications: IHC, Western blot, ELISA
Product
CACNA2D2 Antibody (aa643‑671, Azide‑free, HRP) LS‑C253288
Species: Mouse, Human
Applications: IHC, Western blot, ELISA
CACNA2D2 Antibody - Anti-CACNA2D2 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ CACNA2D2 Antibody (clone 3A4) LS‑B6028
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
CACNA2D2 Antibody - CACNA2D2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cerebellum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
CACNA2D2 Antibody (aa643‑671) LS‑C168258
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
CACNA2D2 Antibody - CACNA2D2 antibody Western Blot of HCT15. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CACNA2D2 Antibody (aa1030‑1079) LS‑C151175
Species: Human
Applications: Western blot
POLE / POLE1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using POLE1 Antibody. The picture on the right is blocked with the synthesized peptide.
POLE / POLE1 Antibody (aa1261‑1310) LS‑C119909
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
See all CACNA2D2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 7/27/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com