Target
Human CA1 / Carbonic Anhydrase I
Synonyms
CA1 | CAB | Carbonic anhydrase 1 | CAR1 | Carbonate dehydratase I | Carbonic dehydratase | CA-I | Carbonic anhydrase B | Carbonic anhydrase I
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Carbonic Anhydrase I [Human Erythrocytes ].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ]. Cross reactivity against Carbonic Anhydrase I from other tissues and species may occur but have not been specifically determined.
Applications
- IHC (1:500 - 1:2500)
- Western blot (1:1000 - 1:5000)
- ELISA (1:5000 - 1:20000)
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Usage
Anti-Carbonic Anhydrase I is useful in ELISA, western blot, and Immunohistochemistry. Optimal titers should be obtained by researchers. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CA1 / Carbonic Anhydrase I