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Antibodies > c-CBL > LS-C351945
 
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Catalog Number Size Price
LS-C351945-100 100 µl (1 mg/ml) $315 
c-CBL Antibody - Immunohistochemical analysis of c-CBL (pY774) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
c-CBL Antibody - Immunofluorescent analysis of c-CBL (pY774) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
c-CBL Antibody - Western blot analysis of c-CBL (pY774) expression in Jurkat (A); mouse liver (B); rat liver (C) whole cell lysates.
c-CBL Antibody - Immunohistochemical analysis of c-CBL (pY774) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
c-CBL Antibody - Immunofluorescent analysis of c-CBL (pY774) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
c-CBL Antibody - Western blot analysis of c-CBL (pY774) expression in Jurkat (A); mouse liver (B); rat liver (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human c‑CBL Antibody (phospho‑Tyr774, IHC, IF, WB) LS‑C351945

Polyclonal Rabbit anti‑Human c‑CBL Antibody (phospho‑Tyr774, IHC, IF, WB) LS‑C351945

Submit Review LSBio Guarantee
Antibody:
c-CBL Rabbit anti-Human Polyclonal (pTyr774) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C351945-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
c-CBL Rabbit anti-Human Polyclonal (pTyr774) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
C-CBL antibody LS-C351945 is an unconjugated rabbit polyclonal antibody to c-CBL (pTyr774) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human c-CBL
Synonyms
CBL | C-CBL | Oncogene CBL2 | RING finger protein 55 | Proto-oncogene c-Cbl | RNF55 | CBL2 | FRA11B | NSLL
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human c-CBL.
Epitope
pTyr774
Specificity
Recognizes endogenous levels of c-CBL (pY774) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About c-CBL
P22681 NM_005188 NP_005179.2

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Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
c-CBL Antibody - Immunohistochemical analysis of c-CBL (pY700) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
c‑CBL Antibody (C‑Terminus) LS‑C358440
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
c-CBL Antibody - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using CBL Antibody. The picture on the right is blocked with the synthesized peptide.
c‑CBL Antibody (aa740‑789) LS‑C117634
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
c-CBL Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CBL Antibody. The picture on the right is blocked with the synthesized peptide.
c‑CBL Antibody (aa666‑715) LS‑C117635
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
c-CBL Antibody - Staining (2 ug/ml) of 3T3 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
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Species: Human, Monkey, Mouse, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all c-CBL Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/9/2025
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