Target
Mouse C/EBP Delta / CEBPD
Synonyms
CEBPD | C/EBP-delta | C/EBP delta | CELF | CRP3 | NF-IL6-beta | Nuclear factor NF-IL6-beta
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
Specificity
This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.
Applications
- IHC (1:2000)
- Western blot (1:1000 - 1:15000)
- Immunoprecipitation (0.8 - 1.5 µg/ml)
- ELISA (1:4000 - 1:9000)
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Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kD in size corresponding to C/EBPS by western blotting in the appropriate cell lysate or extract. The predicted molecular weight of mouse C/EBPS is 28.8 kD. This antibody is capable of detecting both over-expressed and endogenous C/EBPS.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About C/EBP Delta / CEBPD