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Antibodies > C/EBP Beta / CEBPB > LS-C344026
 
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Catalog Number Size Price
LS-C344026-10 10 µg $318 
LS-C344026-100 100 µg (0.5 mg/ml) $470 
C/EBP Beta / CEBPB Antibody - CEBP Beta antibody IHC-paraffin: Human Placenta Tissue.
C/EBP Beta / CEBPB Antibody - Western blot analysis of CEBP Beta using anti-CEBP Beta antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP Beta antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEBP Beta at approximately 46KD. The expected band size for CEBP Beta is at 36KD.
C/EBP Beta / CEBPB Antibody - Western blot analysis of CEBP Beta using anti-CEBP Beta antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP Beta antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEBP Beta at approximately 42-46KD. The expected band size for CEBP Beta is at 36KD.
C/EBP Beta / CEBPB Antibody - CEBP Beta antibody Western blot. All lanes: Anti CEBP Beta at 0.5 ug/ml. WB: Recombinant Human CEBP Beta Protein 0.5ng. Predicted band size: 42 kD. Observed band size: 42 kD.
C/EBP Beta / CEBPB Antibody - CEBP Beta antibody IHC-paraffin: Human Placenta Tissue.
C/EBP Beta / CEBPB Antibody - Western blot analysis of CEBP Beta using anti-CEBP Beta antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP Beta antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEBP Beta at approximately 46KD. The expected band size for CEBP Beta is at 36KD.
C/EBP Beta / CEBPB Antibody - Western blot analysis of CEBP Beta using anti-CEBP Beta antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP Beta antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEBP Beta at approximately 42-46KD. The expected band size for CEBP Beta is at 36KD.
C/EBP Beta / CEBPB Antibody - CEBP Beta antibody Western blot. All lanes: Anti CEBP Beta at 0.5 ug/ml. WB: Recombinant Human CEBP Beta Protein 0.5ng. Predicted band size: 42 kD. Observed band size: 42 kD.
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Polyclonal Rabbit anti‑Human C/EBP Beta / CEBPB Antibody (aa1‑200, IHC, WB) LS‑C344026

Polyclonal Rabbit anti‑Human C/EBP Beta / CEBPB Antibody (aa1‑200, IHC, WB) LS‑C344026

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Note: This antibody replaces LS-C388106
Antibody:
C/EBP Beta / CEBPB Rabbit anti-Human Polyclonal (aa1-200) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344026-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
C/EBP Beta / CEBPB Rabbit anti-Human Polyclonal (aa1-200) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CEBPB antibody LS-C344026 is an unconjugated rabbit polyclonal antibody to CEBPB (C / EBP Beta) (aa1-200) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human C/EBP Beta / CEBPB
Synonyms
CEBPB | C/EBP-beta | C/EBP beta | CRP2 | LAP | Liver activator protein | Nuclear factor NF-IL6 | IL6DBP | TCF5 | NFIL6 | TCF-5 | Transcription factor 5 | NF-IL6
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CEBP Beta recombinant protein (Position: M1-A200). Human CEBP Beta shares 61% amino acid (aa) sequence identity with both mouse and rat CEBP Beta.
Epitope
aa1-200
Specificity
Expressed at low levels in the lung, kidney and spleen.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About C/EBP Beta / CEBPB
P17676 NM_005194 NP_005185.2

LSBio Ratings

C/EBP Beta / CEBPB Antibody (aa1-200) for IHC, WB/Western LS-C344026 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

Deubiquitinase USP1 enhances CCAAT/enhancer-binding protein beta (C/EBPß) stability and accelerates adipogenesis and lipid accumulation. Myung Sup Kim , Jung-Hwan Baek , JinAh Lee, Aneesh Sivaraman, Kyeong Lee, Kyung-Hee Chun. Cell death & disease. 2023 November;14:776.
Full Text Article PubMed: 38012162 PMC: PMC10681981
Submit a Publication for LS-C344026

Customer Reviews (0)

Submit a review for LS-C344026

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IHC‑plus™ C/EBP Beta / CEBPB Antibody (aa39‑78, clone 21A1) LS‑B1688
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
C/EBP Beta / CEBPB Antibody - Anti-CEBPB / CEBP Beta antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ C/EBP Beta / CEBPB Antibody (clone 47A1) LS‑B2926
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA, Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
C/EBP Beta / CEBPB Antibody - P388D1 (IL-1) cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP beta antibody (Clone 1H7).
C/EBP Beta / CEBPB Antibody (clone 1H7) LS‑C41102
Species: Mouse, Rat
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Product
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Species: Human
Applications: Western blot, ELISA
C/EBP Beta / CEBPB Antibody - Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using C/EBP-beta Antibody. The picture on the right is blocked with the synthesized peptide.
C/EBP Beta / CEBPB Antibody (aa201‑250) LS‑C117552
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
C/EBP Beta / CEBPB Antibody - Western blot analysis of lysates from Jurkat cells, using CEBPB Antibody. The lane on the right is blocked with the synthesized peptide.
C/EBP Beta / CEBPB Antibody (aa231‑280) LS‑C118802
Species: Human, Mouse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all C/EBP Beta / CEBPB Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/15/2024
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