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Catalog Number Size Price
LS-C330834-20 20 µl $275 
LS-C330834-100 100 µl $389 
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded mouse kidney using BMI1 antibody at dilution of 1:200 (x400 lens).
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded Human gastric cancer tissue.
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded mouse brain using BMI1 antibody at dilution of 1:200 (x400 lens).
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded human esophageal cancer using BMI1 antibody at dilution of 1:200 (x400 lens).
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded human stomach using BMI1 antibody at dilution of 1:200 (x400 lens).
BMI1 / PCGF4 Antibody - Immunofluorescence analysis of HeLa cell using BMI1 antibody. Blue: DAPI for nuclear staining.
BMI1 / PCGF4 Antibody - Western blot analysis of extracts of various cell lines, using BMI1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
BMI1 / PCGF4 Antibody - Western blot analysis of extracts of K562 cells using BMI1 antibody.
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded mouse kidney using BMI1 antibody at dilution of 1:200 (x400 lens).
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded Human gastric cancer tissue.
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded mouse brain using BMI1 antibody at dilution of 1:200 (x400 lens).
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded human esophageal cancer using BMI1 antibody at dilution of 1:200 (x400 lens).
BMI1 / PCGF4 Antibody - Immunohistochemistry of paraffin-embedded human stomach using BMI1 antibody at dilution of 1:200 (x400 lens).
BMI1 / PCGF4 Antibody - Immunofluorescence analysis of HeLa cell using BMI1 antibody. Blue: DAPI for nuclear staining.
BMI1 / PCGF4 Antibody - Western blot analysis of extracts of various cell lines, using BMI1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
BMI1 / PCGF4 Antibody - Western blot analysis of extracts of K562 cells using BMI1 antibody.
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Polyclonal Rabbit anti‑Human BMI1 / PCGF4 Antibody (IHC, IF, WB) LS‑C330834

Polyclonal Rabbit anti‑Human BMI1 / PCGF4 Antibody (IHC, IF, WB) LS‑C330834

Antibody:
BMI1 / PCGF4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C330834-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
BMI1 / PCGF4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PCGF4 antibody LS-C330834 is an unconjugated rabbit polyclonal antibody to human PCGF4 (BMI1). Validated for IF, IHC and WB.
Target
Human BMI1 / PCGF4
Synonyms
BMI1 | BMI-1 | Flvi-2/bmi-1 | FLVI2/BMI1 | PCGF4 | Polycomb group protein Bmi1 | Polycomb complex protein BMI-1 | Dna-binding protein bmi-1 | Polycomb group ring finger 4 | RING finger protein 51 | RNF51
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 120-326 of human BMI1 (NP_005171.4). EDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG
Specificity
Human BMI1 / PCGF4
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 36kDa, while the observed MW by Western blot was 42kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BMI1 / PCGF4
P35226 NM_005180 NP_005171.4

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024