Target
Mouse BMI1 / PCGF4
Synonyms
BMI1 | BMI-1 | Flvi-2/bmi-1 | FLVI2/BMI1 | PCGF4 | Polycomb group protein Bmi1 | Polycomb complex protein BMI-1 | Dna-binding protein bmi-1 | Polycomb group ring finger 4 | RING finger protein 51 | RNF51
Reactivity
Mouse, Human, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Recombinant Bmi-1 protein corresponding to aa1-202 of mouse Bmi-1.
Specificity
Recognizes mouse Bmi-1 (triplet), Mr 40-44kD. Species cross-reactivity: human, rat and rabbit.
Applications
- ICC
- Western blot (0.2 - 2 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate Bmi-1 from mouse embryo protein extracts. Immunocytochemistry: Reported to immunostain U2OS cells fixed with 2% formaldehyde. Positive control: USOS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee