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Catalog Number Size Price
LS-C153974-2 2 ml (85 mg/ml) $417 
Beta Galactosidase Antibody - Anti-B-Galactosidase Antibody - Western Blot. Western blotting using Fluorescein conjugated anti-b-Galactosidase antibody shows a band at ~117 kD (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blot. The membrane was probed with fluorescein conjugated anti-b-Galactosidase ( diluted to 1:10000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by BIO-RAD. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑E. coli Beta Galactosidase Antibody (IHC, WB) LS‑C153974

Polyclonal Rabbit anti‑E. coli Beta Galactosidase Antibody (IHC, WB) LS‑C153974

Antibody:
Beta Galactosidase Rabbit anti-E. coli Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Unmodified
Price
Catalog Number
$417
LS-C153974-2
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Beta Galactosidase Rabbit anti-E. coli Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Galactosidase antibody LS-C153974 is an unconjugated rabbit polyclonal antibody to e. coli Beta Galactosidase. Validated for ELISA, IHC and WB.
Target
E. coli Beta Galactosidase
Host
Rabbit
Reactivity
E. coli (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
Beta Galactosidase (E.coli)
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Beta Galactosidase [E. coli]. Cross reactivity against Beta Galactosidase from other sources may occur but have not been specifically determined.
Applications
  • IHC (1:500)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:65000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/23/2024