E. coli Beta Galactosidase

Rabbit

E. coli (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Unmodified

Full length native Beta Galactosidase isolated from E.coli

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.

• IHC (1:1500)
• Immunofluorescence
• Western blot (1:5000 - 1:10000)
• Immunoprecipitation
• ELISA (1:10000)

Beta-Gal Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:1 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 mu m) to produce free-floating sections.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.