Target
Human BBC3 / PUMA
Synonyms
BBC3 | Bcl-2-binding component 3 | BCL2 binding component 3 | JFY-1 | JFY1 | PUMA | PUMA alpha
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DGGRPQRTVR, from the internal region of the protein sequence according to NP_001120712.1.
Specificity
Human BBC3 / PUMA. This antibody is expected to recognize isoform 1 (NP_001120712.1) only.
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 24kD band observed in Human peripheral blood mononucleocytes lysates (calculated MW of 26.5kD according to NP_001120712.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee