Human BAX

Mouse

Human, Orangutan, Monkey, Dog, Pig (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG1 Monoclonal

2D2

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified or Ready-to-Use.

Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Baboon, Monkey, Ferret, Cat, Pig, Dog (100%); Bat, Elephant (93%).

Human BAX

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Immunoprecipitation
• Flow Cytometry (0.5 - 1 µg/10E6 cells)
• ELISA

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes a protein of 21kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2. ELISA: Order BSA-free format for coating.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q07812 NCBI: NM_004324 NP_004315.1