Target
Human Basigin / Emmprin / CD147
Synonyms
BSG | 5F7 | Basigin | Basigin (Ok blood group) | CD147 antigen | EMMPRIN | OK | OK blood group antigen | TCSF | CD147 | M6
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Allophycocyanin
Purification
Size exclusion chromatography
Immunogen
Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer).
Specificity
The antibody MEM-M6/1 recognizes an epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
Applications
- IHC - Paraffin
- Western blot
- Immunoprecipitation
- Flow Cytometry
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Usage
The reagent is designed for Flow Cytometry analysis of human blood cells using 10 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Presentation
PBS, 15mM sodium azide
Storage
Store at 2°C to 8°C. Do not freeze. Avoid prolonged exposure to light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Basigin / Emmprin / CD147