Target
Human BAFF Receptor / CD268
Synonyms
TNFRSF13C | BAFFR | BR3 | BAFF receptor | BLyS receptor 3 | CD268 | CD268 antigen | CVID4 | BAFF-R | BROMIX
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
R. Phycoerythrin.
Also available
Unconjugated or conjugated with
Biotin,
FITC.
Purification
Protein A purified
Immunogen
Recombinant human CD268.
Specificity
Antibody ANC268.2 binds to recombinant CD268 (but not CD269) in EIA and native CD268 expressed on Raji cell surface.
Applications
- Flow Cytometry
- ELISA
- Applications tested for the base form of this product only
Usage
Five x 105 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD268/R-PE at a dilution factor of 1:50 (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.52 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD268 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAFF Receptor / CD268