Target
Human BACE2
Synonyms
BACE2 | 56 kDa aspartic-like protease | AEPLC | ALP56 | Aspartic-like protease 56 kDa | APL56 | BAE2 | Beta-secretase 2 | ASP21 | CDA13 | DRAP | Memapsin-1 | Asp 1 | ASP1 | Aspartyl protease 1 | Theta-secretase | Beta secretase 2 | CEAP1 | Down region aspartic protease
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
NS0-derived rhBACE-2 (aa63-466).
Specificity
Selected for its ability to recognize human BACE-2 in direct ELISAs and Western Blots. In these formats, this antibody shows less than 1% cross-reactivity with rhBACE-1, rhCathepsin D and rhCathepsin E.
Applications
- Western blot
- Immunoprecipitation
- ELISA
Usage
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human and mouse BACE-2. The detection limit for BACE-2 is approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation: This antibody has been used to immunoprecipitate rhBACE-2 from conditioned media of transfected NS0 cells. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human and mouse BACE-2. The detection limit for BACE-2 is approximately 0.5 ng/well.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee