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Catalog Number Size Price
LS-C775399-100 100 µg (1 mg/ml) $442 
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
AUTL1 / ATG4C Antibody - Western blot analysis of Rat Liver showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
AUTL1 / ATG4C Antibody - Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
AUTL1 / ATG4C Antibody - Western blot analysis of Rat Liver showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
AUTL1 / ATG4C Antibody - Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
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Polyclonal Rabbit anti‑Human AUTL1 / ATG4C Antibody (IF, WB) LS‑C775399

Polyclonal Rabbit anti‑Human AUTL1 / ATG4C Antibody (IF, WB) LS‑C775399

Antibody:
AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$442
LS-C775399-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ATG4C antibody LS-C775399 is an unconjugated rabbit polyclonal antibody to ATG4C (AUTL1) from human. It is reactive with human, mouse and rat. Validated for IF and WB.
Target
Human AUTL1 / ATG4C
Synonyms
ATG4C | AUTL3 | APG4-C | APG4C | AUTL1 | Autophagin-3 | APG4 autophagy 4 homolog C | Cysteine protease ATG4C
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of human ATG4C
Specificity
Predicted molecular weight at ~52.5 kDa. Observed molecular weights at ~45-60 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AUTL1 / ATG4C
Q96DT6 NM_032852 NP_116241.2

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Requested From: United States
Date Requested: 11/3/2024