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Antibodies > ATF1 > LS-C810067
 
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Catalog Number Size Price
LS-C810067-100 100 µl (1 mg/ml) $379 
LS-C810067-200 200 µl (1 mg/ml) $421 
ATF1 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ATF1 Antibody - Western blot analysis of Phospho-ATF1 (Ser63) antibody expression in Insulin treated HT29 cells lysates. The lane on the right is treated with the antigen-specific peptide.
ATF1 Antibody - Western blot analysis of ATF1 phosphorylation expression in Insulin treated HT29 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ATF1 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ATF1 Antibody - Western blot analysis of Phospho-ATF1 (Ser63) antibody expression in Insulin treated HT29 cells lysates. The lane on the right is treated with the antigen-specific peptide.
ATF1 Antibody - Western blot analysis of ATF1 phosphorylation expression in Insulin treated HT29 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human ATF1 Antibody (phospho‑Ser63, IF, WB) LS‑C810067

Polyclonal Rabbit anti‑Human ATF1 Antibody (phospho‑Ser63, IF, WB) LS‑C810067

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Antibody:
ATF1 Rabbit anti-Human Polyclonal (pSer63) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C810067-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ATF1 Rabbit anti-Human Polyclonal (pSer63) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ATF1 antibody LS-C810067 is an unconjugated rabbit polyclonal antibody to ATF1 (pSer63) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
Target
Human ATF1
Synonyms
ATF1 | ATF-1 | EWS-ATF1 | FUS/ATF-1 | Protein TREB36 | TREB36
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human ATF1 around the phosphorylation site of Serine 63.
Epitope
pSer63
Specificity
Phospho-ATF1 (Ser63) Antibody detects endogenous levels of ATF1 only when phosphorylated at Serine 63.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATF1
P18846 NM_005171 NP_005162.1

Publications (0)

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
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Tel: (800) 227-6666 / (650) 697-3600

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