Human ARSD / Arylsulfatase D

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-DASNGYRALQWNA, from the internal region of the protein sequence according to NP_001660.2NP_033667.2.

aa108-120

Human ARSD / Arylsulfatase D. This antibody is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2)

• Western blot (0.3 - 1 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:16000)

Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 60+40kD bands observed in lysates of cell lines A549 and HepG2 (calculated MW of 64.9kD according to NP_001660.2 and of 42.3 according to NP_033667.2). Recommended concentration: 0.3-1 ug/ml. An additional band of unknown identity was also consistently observed at 35kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P51689 NCBI: NM_009589 NP_033667.2