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Catalog Number	Size	Price
LS-C407688-10	10 µg	$318
LS-C407688-100	100 µg	$470

ARSA / Arylsulfatase A Antibody - ARSA antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

ARSA / Arylsulfatase A Antibody - ARSA antibody IHC-paraffin. IHC(P): Rat Lung Tissue.

ARSA / Arylsulfatase A Antibody - IF analysis of ARSA using anti-ARSA antibody ARSA was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-ARSA Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

ARSA / Arylsulfatase A Antibody - ARSA antibody Western blot. All lanes: Anti ARSA at 0.5 ug/ml. Lane 1: Rat Testis Tissue Lysate at 50 ug. Lane 2: Rat Pancreas Tissue Lysate at 50 ug. Lane 3: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 4: Mouse Kidney Tissue Lysate at 50 ug. Lane 5: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 54 kD. Observed band size: 54 kD.

ARSA / Arylsulfatase A Antibody - Flow Cytometry analysis of PC-3 cells using anti-ARSA antibody. Overlay histogram showing PC-3 cells stained with anti-ARSA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARSA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

ARSA / Arylsulfatase A Antibody - Flow Cytometry analysis of Hela cells using anti-ARSA antibody. Overlay histogram showing Hela cells stained with anti-ARSA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARSA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human ARSA / Arylsulfatase A Antibody (aa454‑482, IHC, WB) LS‑C407688

Name: Anti-ARSA / Arylsulfatase A Antibody | Rabbit anti-Human IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407688-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

ARSA / Arylsulfatase A Rabbit anti-Human Polyclonal (aa454-482) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C407688-10

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

ARSA / Arylsulfatase A Rabbit anti-Human Polyclonal (aa454-482) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Arylsulfatase A antibody LS-C407688 is an unconjugated rabbit polyclonal antibody to Arylsulfatase A (ARSA) (aa454-482) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.

Target

Human ARSA / Arylsulfatase A

Synonyms

ARSA | Arylsulfatase A | Cerebroside-sulfatase | MLD | ASA

See All ARSA / Arylsulfatase A Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human ARSA (454-482 aa QALKQLQLLKAQLDAAVTFGPSQVARGED), different from the related mouse sequence by six amino acids.

Epitope

aa454-482

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ARSA / Arylsulfatase A

Uniprot: P15289 NCBI: NM_000487 NP_000478.3