Target
Human ARNT / HIF-1-Beta
Synonyms
ARNT | ARNT protein | BHLHe2 | HIF-1beta | HIF1-beta | HIF1BETA | TANGO | HIF-1-beta | HIF1B
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence CNDKERFARSDDEQS, from the internal region (near N-Terminus) of the protein sequence according to NP_001659.1;.
Specificity
Human ARNT / HIF-1-Beta. This antibody is expected to recognize all reported isoform 1 (NP_001659.1) only.
Applications
- Western blot (2 - 4 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 90kD band observed in nuclear lysates of cell lines HeLa and HepG2 (calculated MW of 86.6kD according to NP_001659.1). Recommended concentration: 2-4 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee