Target
Human ARNT / HIF-1-Beta
Synonyms
ARNT | ARNT protein | BHLHe2 | HIF-1beta | HIF1-beta | HIF1BETA | TANGO | HIF-1-beta | HIF1B
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-SEIYHNINADQSK, from the internal region of the protein sequence according to NP_001659.1NP_848514.1NP_001184254.1.
Specificity
Human ARNT / HIF-1-Beta. This antibody is expected to recognize all reported isoforms (NP_001659.1; NP_848514.1; NP_001184254.1).
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 100kD band observed in nuclear lysates of cell line HeLa (calculated MW of 86.6kD according to NP_001659.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee