Target
Mouse ARHGAP22 / RhoGAP2
Synonyms
ARHGAP22 | RhoGAP2 | RhoGap22
Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS397 region of ARHGAP22.
Specificity
This antibody is specific for phosphorylated ARHGAP22 at Serine 397. It also recognizes the S22->A mutation but not the S397->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS397 from other sources has not been determined.
Applications
- IHC (1:100 - 1:500)
- Immunofluorescence (1:100 - 1:500)
- Western blot (1 µg/ml)
- ELISA (1:20000 - 1:60000)
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Usage
Anti-ARHGAP22 pS397 antibody is useful for ELISA, Western Blot and Immunostaining. Expect a band approximately ~77.8 kD corresponding to the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 1 mg/ml BSA, 50% Glycerol
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee