Human ARA55 / HIC-5

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with Biotin, FITC, HRP.

Immunoaffinity purified

Unmodified

Synthetic peptide from N-Terminus of human TGFB1I1 (B2R8D5, NP_057011). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Panda, Dog, Rabbit (100%); Marmoset, Horse (92%); Bat (84%).

N-Terminus

Human TGFB1I1 / HIC-5

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.2 - 1 µg/ml)

Immunohistochemistry: LS-B3528 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3528 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Tested in Western blot using transfected cells. ELISA titer in peptide based assay: 1:312500.

PBS, 0.09% sodium azide, 2% sucrose

Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O43294 NCBI: NM_015927 NP_057011.2