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Catalog Number Size Price
LS-C413484-100 100 µl (1 mg/ml) $321 
AQP4 / Aquaporin 4 Antibody - Immunohistochemical analysis of Aquaporin 4 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
AQP4 / Aquaporin 4 Antibody - Immunofluorescent analysis of Aquaporin 4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
AQP4 / Aquaporin 4 Antibody - Western blot analysis of Aquaporin 4 expression in HeLa (A); mouse heart (B); rat heart (C) whole cell lysates.
AQP4 / Aquaporin 4 Antibody - Immunohistochemical analysis of Aquaporin 4 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
AQP4 / Aquaporin 4 Antibody - Immunofluorescent analysis of Aquaporin 4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
AQP4 / Aquaporin 4 Antibody - Western blot analysis of Aquaporin 4 expression in HeLa (A); mouse heart (B); rat heart (C) whole cell lysates.
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Monoclonal Mouse anti‑Human AQP4 / Aquaporin 4 Antibody (IHC, IF, WB) LS‑C413484

Monoclonal Mouse anti‑Human AQP4 / Aquaporin 4 Antibody (IHC, IF, WB) LS‑C413484

Antibody:
AQP4 / Aquaporin 4 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$321
LS-C413484-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
AQP4 / Aquaporin 4 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Aquaporin 4 antibody LS-C413484 is an unconjugated mouse monoclonal antibody to Aquaporin 4 (AQP4) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB. Cited in 1 publication.
Target
Human AQP4 / Aquaporin 4
Synonyms
AQP4 | Aquaporin type4 | Aquaporin 4 | Aquaporin-4 | HMIWC2 | MIWC | WCH4 | AQP-4
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Aquaporin 4.
Specificity
Recognizes endogenous levels of Aquaporin 4 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:200)
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:1000 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AQP4 / Aquaporin 4
P55087 NM_001650 NP_001641.1

LSBio Ratings

AQP4 / Aquaporin 4 Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western LS-C413484 has an LSBio Rating of
Publications (4)

Publications (1)

Fractional anisotropy from diffusion tensor imaging correlates with acute astrocyte and myelin swelling in neonatal swine models of excitotoxic and hypoxic-ischemic brain injury. Jennifer K Lee, Dapeng Liu, Dengrong Jiang, Ewa Kulikowicz, Aylin Tekes, Peiying Liu, Qin Qin, Raymond C Koehler, Manisha Aggarwal, Jiangyang Zhang, Lee J Martin. The Journal of comparative neurology. 2021 July;529:2750-2770.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/12/2024