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Catalog Number Size Price
LS-C312645-10 10 µg $318 
LS-C312645-100 100 µg (0.5 mg/ml) $470 
AQP3 / Aquaporin 3 Antibody - IHC analysis of Aquaporin 3 using anti-Aquaporin 3 antibody. Aquaporin 3 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Aquaporin 3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
AQP3 / Aquaporin 3 Antibody - IHC analysis of Aquaporin 3 using anti-Aquaporin 3 antibody. Aquaporin 3 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Aquaporin 3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
AQP3 / Aquaporin 3 Antibody - AQP3 / Aquaporin 3 antibody. IHC(F): Rat Kidney Tissue.
AQP3 / Aquaporin 3 Antibody - AQP3 / Aquaporin 3 antibody. IHC(P): Rat Kidney Tissue.
AQP3 / Aquaporin 3 Antibody - AQP3 / Aquaporin 3 antibody. ICC: HELA Cell.
AQP3 / Aquaporin 3 Antibody - WB of AQP3 / Aquaporin 3 antibody. All lanes: Anti-AQP3 at 0.5ug/ml. Lane 1: Rat Kidney Tissue Lysate at 40ug. Lane 2: Rat Lung Tissue Lysate at 40ug. Lane 3: Mouse Kidney Tissue Lysate at 40ug. Lane 4: M453 Whole Cell Lysate at 40ug. Lane 5: MMC Whole Cell Lysate at 40ug. Predicted bind size: 32KD. Observed bind size: 32KD.
AQP3 / Aquaporin 3 Antibody - Flow Cytometry analysis of Hela cells using anti-Aquaporin 3 antibody. Overlay histogram showing Hela cells stained with anti-Aquaporin 3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
AQP3 / Aquaporin 3 Antibody - IHC analysis of Aquaporin 3 using anti-Aquaporin 3 antibody. Aquaporin 3 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Aquaporin 3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
AQP3 / Aquaporin 3 Antibody - IHC analysis of Aquaporin 3 using anti-Aquaporin 3 antibody. Aquaporin 3 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Aquaporin 3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
AQP3 / Aquaporin 3 Antibody - AQP3 / Aquaporin 3 antibody. IHC(F): Rat Kidney Tissue.
AQP3 / Aquaporin 3 Antibody - AQP3 / Aquaporin 3 antibody. IHC(P): Rat Kidney Tissue.
AQP3 / Aquaporin 3 Antibody - AQP3 / Aquaporin 3 antibody. ICC: HELA Cell.
AQP3 / Aquaporin 3 Antibody - WB of AQP3 / Aquaporin 3 antibody. All lanes: Anti-AQP3 at 0.5ug/ml. Lane 1: Rat Kidney Tissue Lysate at 40ug. Lane 2: Rat Lung Tissue Lysate at 40ug. Lane 3: Mouse Kidney Tissue Lysate at 40ug. Lane 4: M453 Whole Cell Lysate at 40ug. Lane 5: MMC Whole Cell Lysate at 40ug. Predicted bind size: 32KD. Observed bind size: 32KD.
AQP3 / Aquaporin 3 Antibody - Flow Cytometry analysis of Hela cells using anti-Aquaporin 3 antibody. Overlay histogram showing Hela cells stained with anti-Aquaporin 3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human AQP3 / Aquaporin 3 Antibody (aa278‑292, IHC, WB) LS‑C312645

Polyclonal Rabbit anti‑Human AQP3 / Aquaporin 3 Antibody (aa278‑292, IHC, WB) LS‑C312645

Note: This antibody replaces LS-C388010
Antibody:
AQP3 / Aquaporin 3 Rabbit anti-Human Polyclonal (aa278-292) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312645-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
AQP3 / Aquaporin 3 Rabbit anti-Human Polyclonal (aa278-292) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Aquaporin 3 antibody LS-C312645 is an unconjugated rabbit polyclonal antibody to Aquaporin 3 (AQP3) (aa278-292) from human. It is reactive with human and rat. Validated for ICC, IHC and WB.
Target
Human AQP3 / Aquaporin 3
Synonyms
AQP3 | Aquaporin 3 (GIL blood group) | Aquaglyceroporin-3 | AQP-3 | Aquaporin 3 | Aquaporin 3 (Gill blood group) | Aquaporin-3 | GIL
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Aquaporin 3(278-292 aa EENVKLAHVKHKEQI), different from the related rat and mouse sequences by one amino acid.
Epitope
aa278-292
Specificity
Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AQP3 / Aquaporin 3
Q92482 NM_004925 NP_004916.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024