Target
Human APOL1 / Apolipoprotein L
Synonyms
APOL1 | Apolipoprotein L-I | APOL-I | Apolipoprotein L, 1 | APOL | Apolipoprotein L | FSGS4 | APO-L | Apolipoprotein L1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-PHASASRPRVTEP, from the internal region of the protein sequence according to NP_003652.2NP_663318.1NP_001130013.1.
Specificity
Human APOL1 / Apolipoprotein L 1. This antibody is expected to recognize all three reported isoforms (NP_003652.2; NP_663318.1; NP_001130013.1). Reported variants represent identical protein: NP_003652.2, NP_001130012.1
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 42+45kD bands observed in Human Placenta lysates (calculated MW of 45.9kD and 42.2kD according to NP_663318.1 and NP_001130013.1 resp.). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOL1 / Apolipoprotein L