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Antibodies > APOA1 / Apolipoprotein A 1 > LS-C355402
 
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Catalog Number Size Price
LS-C355402-1 1 mg $857 
APOA1 / Apolipoprotein A 1 Antibody - Apolipoprotein A-1 Immunohistochemistry
APOA1 / Apolipoprotein A 1 Antibody - Immunofluorescence analysis of Apolipoprotein A1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein A1 (513) Mouse Monoclonal Antibody. Proteins were transferred to a Nitrocellulose Membrane. Proteins were transferred to a Nitrocellulose Membrane using the G2 Fast Blotter, and blocked with 5% milk in TBST for at least 1 hour at room temperature. Apo A-1 was detected at ~50kD using an Apolipoprotein A-1 monoclonal antibody at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG Fc-specific secondary antibody at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura.
APOA1 / Apolipoprotein A 1 Antibody - Apolipoprotein A-1 Immunohistochemistry
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit oligoclonal antibody at a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a biotinylated rabbit anti-mouse IgG Superclonal secondary antibody was incubated for 1 hour, followed by Streptavidin-HRP for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit monoclonal antibody at a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a biotinylated rabbit anti-mouse IgG Superclonal secondary antibody was incubated for 1 hour, followed by Streptavidin-HRP for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit oligoclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 or recombinant mouse Apo A-1 was added to the plate at concentrations ranging from 1.6-1000ng/ml and incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of an HRP-conjugated rabbit anti-mouse IgG cross-adsorbed secondary antibody was incubated for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
APOA1 / Apolipoprotein A 1 Antibody - Apolipoprotein A-1 Immunohistochemistry
APOA1 / Apolipoprotein A 1 Antibody - Immunofluorescence analysis of Apolipoprotein A1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein A1 (513) Mouse Monoclonal Antibody. Proteins were transferred to a Nitrocellulose Membrane. Proteins were transferred to a Nitrocellulose Membrane using the G2 Fast Blotter, and blocked with 5% milk in TBST for at least 1 hour at room temperature. Apo A-1 was detected at ~50kD using an Apolipoprotein A-1 monoclonal antibody at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG Fc-specific secondary antibody at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura.
APOA1 / Apolipoprotein A 1 Antibody - Apolipoprotein A-1 Immunohistochemistry
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit oligoclonal antibody at a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a biotinylated rabbit anti-mouse IgG Superclonal secondary antibody was incubated for 1 hour, followed by Streptavidin-HRP for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit monoclonal antibody at a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a biotinylated rabbit anti-mouse IgG Superclonal secondary antibody was incubated for 1 hour, followed by Streptavidin-HRP for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
APOA1 / Apolipoprotein A 1 Antibody - Sandwich ELISA of Apolipoprotein A-1 was performed by coating wells of a 96-well plate with 100ul of an Apo A-1 rabbit oligoclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 150ul of StartingBlock T20 (TBS) Blocking Buffer for 30 minutes, and 80ul of recombinant human Apo A-1 or recombinant mouse Apo A-1 was added to the plate at concentrations ranging from 1.6-1000ng/ml and incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Apo A-1 mouse monoclonal antibody was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of an HRP-conjugated rabbit anti-mouse IgG cross-adsorbed secondary antibody was incubated for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.
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Monoclonal Mouse anti‑Human APOA1 / Apolipoprotein A 1 Antibody (clone 513, IHC, IF, WB) LS‑C355402

Monoclonal Mouse anti‑Human APOA1 / Apolipoprotein A 1 Antibody (clone 513, IHC, IF, WB) LS‑C355402

Submit Review LSBio Guarantee
Antibody:
APOA1 / Apolipoprotein A 1 Mouse anti-Human Monoclonal (513) Antibody
Application:
IHC, IF, WB, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355402-1
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APOA1 / Apolipoprotein A 1 Mouse anti-Human Monoclonal (513) Antibody
Application:
IHC, IF, WB, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Apolipoprotein A 1 antibody LS-C355402 is an unconjugated mouse monoclonal antibody to human Apolipoprotein A 1 (APOA1). Validated for ELISA, IF, IHC, RIA and WB.
Target
Human APOA1 / Apolipoprotein A 1
Synonyms
APOA1 | ApoA-I | APOAI | Apolipoprotein A-I | Apolipoprotein A1 | Apo-AI
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
513
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Purified human plasma Apo A-1.
Specificity
The antibody targets Apolipoprotein A-1 in ELISA and RIA applications and shows reactivity with Human samples. It detects Apolipoprotein A-1 which has a predicted molecular weight of approximately 29 kD.
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • ELISA
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.015 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOA1 / Apolipoprotein A 1
P02647 NM_000039 NP_000030.1

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Featured Products

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IHC‑plus™ APOA1 / Apolipoprotein A 1 Antibody LS‑B3641
Species: Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
APOA1 / Apolipoprotein A 1 Antibody - Anti-Apolipoprotein A I antibody IHC of mouse kidney, adipose. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ APOA1 / Apolipoprotein A 1 Antibody LS‑B5257
Species: Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Immunodiffusion
APOA1 / Apolipoprotein A 1 Antibody - Anti-Apolipoprotein A I antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ APOA1 / Apolipoprotein A 1 Antibody (aa188‑199) LS‑B2097
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
APOA1 / Apolipoprotein A 1 Antibody - Anti-Apolipoprotein A I antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ APOA1 / Apolipoprotein A 1 Antibody LS‑B2475
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Product
APOA1 / Apolipoprotein A 1 Antibody (N‑Terminus, Magnetic beads) LS‑C172079
Species: Human, Mouse, Rat
Applications: Immunoprecipitation
See all APOA1 / Apolipoprotein A 1 Antibodies

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/4/2025
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6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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