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Catalog Number	Size	Price
LS-C800511-100	100 µl (1 mg/ml)	$379
LS-C800511-200	200 µl (1 mg/ml)	$421

APG4B / ATG4B Antibody - 1:100 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

APG4B / ATG4B Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

APG4B / ATG4B Antibody - Western blot analysis on Jurkat cell lysates using ATG4B antibody. The lane on the left is treated with the antigen-specific peptide.

APG4B / ATG4B Antibody - Western blot analysis of ATG4B expression in mouse brain lysate

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Polyclonal Rabbit anti‑Human APG4B / ATG4B Antibody (IHC, IF, WB) LS‑C800511

Name: Anti-APG4B / ATG4B Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C800511-100
Price: 379.0000 USD
Availability: InStock

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Antibody:

APG4B / ATG4B Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$379

LS-C800511-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

APG4B / ATG4B Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

ATG4B antibody LS-C800511 is an unconjugated rabbit polyclonal antibody to ATG4B (APG4B) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.

Target

Human APG4B / ATG4B

Synonyms

See All APG4B / ATG4B Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Peptide affinity chromatography.

Modifications

Unmodified

Immunogen

Synthesized peptide derived from human Atg4b

Specificity

ATG4B Antibody detects endogenous levels of ATG4B.

Applications

IHC (1:50 - 1:200)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:2000)
Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.

Presentation

PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About APG4B / ATG4B