Target
Human APG12 / ATG12
Synonyms
ATG12 | Autophagy-related protein 12 | APG12L | Autophagy related 12 | FBR93 | APG12 | APG12-like | Ubiquitin-like protein ATG12 | HAPG12
Reactivity
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
Synthetic peptide corresponding to residues surrounding Ser36 of human Atg12 protein (KLH- coupled).
Specificity
Recognizes human endogenous levels of total free and Atg5 bound Atg12 protein. Species cross-reactivity: mouse, rat, monkey.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:100)
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%. Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee