Yeast APG12 / ATG12

Rabbit

Yeast (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Unmodified

This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

• Western blot (1:500 - 1:2000)
• ELISA (1:1000 - 1:5000)

This purified polyclonal antibody reacts yeast APG 12 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O94817 NCBI: NM_004707 NP_004698.3