Human APEX1 / APE1

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Affinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein.

Specific to the human APE/ref-1protein. Competitively inhibited from recognizing the APE/ref-1 antigen in tissues using APE/ref-1 protein. Inhibits repair activity of ape protein.

• IHC
• IHC - Paraffin (1:250)
• IHC - Frozen
• Western blot (1:500)

Immunohistochemistry: LS-B3179 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3179 was determined to be 1:250. Western Blot: 1:500 (ECL). Detects a single band at 37kD. Diluted antibody can be reused 1-3 times in Western Blot analysis. Apoptosis: Can be used following the apoptosis (TUNEL) procedure using the Boehringer-Mannheim TUNEL assay kit. Antibody staining should be performed AFTER the TUNEL assay. Positive control: HeLa whole cell extract.

PBS, pH 7.2, 0.05% Sodium Azide, 40% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P27695 NCBI: NM_001641 NP_001632.2