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Antibodies > APC > LS-C800672
 
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Catalog Number Size Price
LS-C800672-100 100 µl (1 mg/ml) $379 
LS-C800672-200 200 µl (1 mg/ml) $421 
APC Antibody - 1:100 staining human Rectum tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
APC Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
APC Antibody - Western blot analysis on HuvEc cell lysates using Phospho-APC(Ser2054) antibody. The lane on the left is treated with the antigen-specific peptide.
APC Antibody - 1:100 staining human Rectum tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
APC Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
APC Antibody - Western blot analysis on HuvEc cell lysates using Phospho-APC(Ser2054) antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human APC Antibody (phospho‑Ser2054, IHC, IF, WB) LS‑C800672

Polyclonal Rabbit anti‑Human APC Antibody (phospho‑Ser2054, IHC, IF, WB) LS‑C800672

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Antibody:
APC Rabbit anti-Human Polyclonal (pSer2054) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800672-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APC Rabbit anti-Human Polyclonal (pSer2054) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
APC antibody LS-C800672 is an unconjugated rabbit polyclonal antibody to human APC (pSer2054). Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human APC
Synonyms
APC | Adenomatosis polyposis coli | Adenomatous polyposis coli | BTPS2 | Deleted in polyposis 2.5 | DP2 | DP2.5 | FPC | GS | Protein APC | PPP1R46 | DP3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human APC around the phosphorylation site of Sersine 2054.
Epitope
pSer2054
Specificity
Phospho-APC(Ser2054) Antibody detects endogenous levels of APC only when phosphorylated at Sersine 2054.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APC
P25054 NM_000038 NP_000029.2

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APC Antibody - Immunohistochemical analysis of APC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
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