Target
Human APC-AAT Complexes
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Immunogen
Recombinant form of human activated protein C (Drotrecogin alfa, Xigris).
Specificity
Has specificity for a conformation-dependent neoepitope that is expressed in activated protein C upon complex-formation with a1-antitrypsin. No reaction is seen to non-complexed a1-antitrypsin and only very little cross reaction to protein C zymogen. Note that specificity is calcium dependent. Reactivity: Reacts strongly with APC-AAT complexes in ELISA. It can be used in sandwich ELISA in combination with a polyclonal anti-protein C antiserum. Note that the conformational neoepitope expressed in the APC-AAT complex can also be expressed in APC coated directly onto a high-binding microtiter plate.
Usage
Suitable for use in ELISA. ELISA: 1:500.
Presentation
PBS, pH 7.2, 15 mM Sodium Azide
Storage
Short term: store at 4°C for up to 2 weeks. Long term: aliquot and store at -20°C or -70°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee