Human ANXA1 / Annexin A1

Goat

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence CQAILDETKGDYEK, from the C-Terminus of protein sequence according to NP_000691.1.

aa324-337

Human ANXA1 / Annexin A1.

• IHC
• IHC - Paraffin (4 µg/ml)
• Western blot (1 - 3 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:4000)

Immunohistochemistry: LS-B3028 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3028 was determined to be 4 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kD according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al, Cancer Res. 2000 Nov15;60(22):6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 ug/ml.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04083 NCBI: NM_000700 NP_000691.1