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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2478-50 | 50 µg (1 mg/ml) | $585 |
IHC‑plus™ Monoclonal Mouse anti‑Human TPSAB1 / Mast Cell Tryptase Antibody (clone AA1, IHC, WB) LS‑B2478
IHC‑plus™ Monoclonal Mouse anti‑Human TPSAB1 / Mast Cell Tryptase Antibody (clone AA1, IHC, WB) LS‑B2478
Note: This antibody replaces LS-C25456
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Mast Cell Tryptase antibody LS-B2478 is an unconjugated mouse monoclonal antibody to Mast Cell Tryptase (TPSAB1) from human. It is reactive with human, cat, dog and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TPSAB1 / Mast Cell Tryptase
Synonyms
TPSAB1 | Alpha tryptase | Mast cell alpha II tryptase | Tryptase alpha-1 | Tryptase beta I | Tryptase-III | TPSB1 | Tryptase alpha II | TPS1 | TPS2 | Tryptase beta-1 | Tryptase-1 | Tryptase-I | Mast cell beta I tryptase | Tryptase alpha/beta 1 | Tryptase alpha/beta-1 | Tryptase beta 1 | Tryptase I
Host
Mouse
Reactivity
Human, Monkey, Cat, Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
AA1
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Human mast cell tryptase purified from human lung tissue.
Specificity
Recognizes human mast cell tryptase, both alpha and beta isoforms. The antibody is an excellent marker for mast cells and does not bind to any other cell type in Immunohistochemistry. Species cross-reactivity: feline, canine and monkey.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- IHC - Frozen
- Western blot
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2478 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2478 was determined to be 5 ug/ml. Positive control: Tonsil.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TPSAB1 / Mast Cell Tryptase
Validation
Anti-TPSAB1 / Mast Cell Tryptase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TPSAB1 / Mast Cell Tryptase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ TPSAB1 / Mast Cell Tryptase Antibody (clone AA1) for IHC, WB/Western, ELISA LS-B2478 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, ELISA
Species:
Mouse
Applications:
Western blot, Immunoprecipitation
Species:
Mouse
Applications:
Western blot, Immunoprecipitation, ELISA, Neutralization
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
Western blot, ELISA
Reactivity:
Human
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/24/2024
Date Requested: 11/24/2024
