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Catalog Number Size Price
LS-B10408-50 50 µl (1 mg/ml) $460 
Rhodopsin / RHO Antibody - Anti-Rhodopsin / RHO antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rhodopsin / RHO Antibody - High magnification confocal image of pig retinal section stained with Rhodopsin / RHO antibody (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).
Rhodopsin / RHO Antibody - Blot of bovine retinal extracts probed with Rhodopsin / RHO antibody. The antibody stains a band corresponding to retinal rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin. Note, due to the highly hydrophobic nature of rhodopsin, it important to avoid boiling samples containing this protein it in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the rhodopsin protein and appearance of more of this high molecular weight material.
Rhodopsin / RHO Antibody - Anti-Rhodopsin / RHO antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rhodopsin / RHO Antibody - High magnification confocal image of pig retinal section stained with Rhodopsin / RHO antibody (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).
Rhodopsin / RHO Antibody - Blot of bovine retinal extracts probed with Rhodopsin / RHO antibody. The antibody stains a band corresponding to retinal rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin. Note, due to the highly hydrophobic nature of rhodopsin, it important to avoid boiling samples containing this protein it in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the rhodopsin protein and appearance of more of this high molecular weight material.
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IHC‑plus™ Monoclonal Mouse anti‑Human Rhodopsin / RHO Antibody (clone B630, IHC, IF, WB) LS‑B10408

IHC‑plus™ Monoclonal Mouse anti‑Human Rhodopsin / RHO Antibody (clone B630, IHC, IF, WB) LS‑B10408

Note: This antibody replaces LS-C204581
Antibody:
Rhodopsin / RHO Mouse anti-Human Monoclonal (B630) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B10408-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Rhodopsin / RHO Mouse anti-Human Monoclonal (B630) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
RHO antibody LS-B10408 is an unconjugated mouse monoclonal antibody to RHO (Rhodopsin) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Rhodopsin / RHO
Synonyms
RHO | CSNBAD1 | OPN2 | Rhodopsin | RP4 | Opsin 2, rod pigment | Opsin-2
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
B630
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Purified rhodopsin from bovine retina
Specificity
Recognizes a peptide corresponding to the N-terminal 32 amino acids
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Try at dilutions of ~1:1000 for immunofluorescence. For western blots try at 1:5000. A suitable control tissue is retinal extracts. Rhodopsin run at 35 kDa on SDS-PAGE gels.
Presentation
PBS, 10 mM Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Rhodopsin / RHO
P08100 NM_000539 NP_000530.1

Validation

Rhodopsin / RHO Antibody - Anti-Rhodopsin / RHO antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Rhodopsin / RHO antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Rhodopsin / RHO antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ Rhodopsin / RHO Antibody (clone B630) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B10408 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2024