Human HES1 / HES-1

Rabbit

Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Rabbit, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide corresponding to aa24-35 (TPDKPKTASEH) OF HES1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%); Newt, Smelt (91%); Chicken (82%).

aa24-35

Recognizes human HES-1 (Hairy family).

• IHC
• IHC - Paraffin (5 µg/ml)
• IHC - Frozen
• Immunofluorescence
• Western blot (1:200 - 1:1000)

Immunohistochemistry: LS-B2211 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2211 was determined to be 5 ug/ml. Positive control: Human neural stem cells, Raji cell lysate.

PBS, pH 7.2, No preservatives added

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q14469 NCBI: NM_005524 NP_005515.1