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Catalog Number Size Price
LS-B3633-200 200 µl $460 
ENO1 / Alpha Enolase Antibody - Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ENO1 / Alpha Enolase Antibody - Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ENO1 / Alpha Enolase Antibody - Fluorescent confocal image of C2C12 cell stained with ENO1 Antibody. C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENO1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENO1 immunoreactivity is localized to Cytoplasm significantly.
ENO1 / Alpha Enolase Antibody - ENO1 Antibody western blot of Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35 ug/lane). The ENO1 antibody detected the ENO1 protein (arrow).
ENO1 / Alpha Enolase Antibody - ENO1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
ENO1 / Alpha Enolase Antibody - Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ENO1 / Alpha Enolase Antibody - Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ENO1 / Alpha Enolase Antibody - Fluorescent confocal image of C2C12 cell stained with ENO1 Antibody. C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENO1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENO1 immunoreactivity is localized to Cytoplasm significantly.
ENO1 / Alpha Enolase Antibody - ENO1 Antibody western blot of Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35 ug/lane). The ENO1 antibody detected the ENO1 protein (arrow).
ENO1 / Alpha Enolase Antibody - ENO1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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IHC‑plus™ Polyclonal Rabbit anti‑Human ENO1 / Alpha Enolase Antibody (aa178‑205, IHC, IF, WB) LS‑B3633

IHC‑plus™ Polyclonal Rabbit anti‑Human ENO1 / Alpha Enolase Antibody (aa178‑205, IHC, IF, WB) LS‑B3633

Note: This antibody replaces LS-C99250
Antibody:
ENO1 / Alpha Enolase Rabbit anti-Human Polyclonal (aa178-205) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3633-200
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ENO1 / Alpha Enolase Rabbit anti-Human Polyclonal (aa178-205) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha Enolase antibody LS-B3633 is an unconjugated rabbit polyclonal antibody to human Alpha Enolase (ENO1) (aa178-205). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ENO1 / Alpha Enolase
Synonyms
ENO1 | Alpha Enolase | Alpha enolase like 1 | ENO1L1 | Enolase 1 | MBP-1 | MBPB1 | MPB1 | MYC promoter-binding protein 1 | NNE | Phosphopyruvate hydratase | Alpha-enolase | Tau-crystallin | MBP1 | MPB-1 | C-myc promoter-binding protein | Enolase 1, (alpha) | Enolase-alpha | Non-neural enolase | Plasminogen-binding protein | PPH
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat, Chicken, Xenopus, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa178-205
Specificity
This ENO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the Central region of human ENO1.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3633 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3633 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ENO1 / Alpha Enolase
P06733 NM_001428 NP_001419.1

Validation

ENO1 / Alpha Enolase Antibody - Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

ENO1 / Alpha Enolase Antibody - Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ ENO1 / Alpha Enolase Antibody (aa178-205) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B3633 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/10/2024