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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2425-50 | 50 µg (1 mg/ml) | $535 |
IHC‑plus™ Monoclonal Rat anti‑Mouse CD34 Antibody (clone MEC14.7, IHC) LS‑B2425
IHC‑plus™ Monoclonal Rat anti‑Mouse CD34 Antibody (clone MEC14.7, IHC) LS‑B2425
Note: This antibody replaces LS-C4946
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
CD34 antibody LS-B2425 is an unconjugated rat monoclonal antibody to CD34 from mouse. It is reactive with human and mouse. Validated for Flow, IHC and IP.
Host
Rat
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
MEC14.7
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Mouse CD34.
Specificity
Recognizes the mouse CD34 cell surface glycoprotein. mouse CD34 is expressed by hematopoietic progenitor cells in the bone marrow and also by endothelial cells.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Immunoprecipitation (2 - 10 µg/ml)
- Flow Cytometry (1:25 - 1:50)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2425 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2425 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD34
Validation
Anti-CD34 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD34 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CD34 Antibody (clone MEC14.7) for IHC, IP, Flow LS-B2425 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Rat
Applications:
IHC, IHC - Frozen, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Non-Human Primates
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
Date Requested: 11/27/2024
