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Catalog Number Size Price
LS-C777147-100 100 µg (1 mg/ml) $479 
ANK1 / Ankyrin Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin R Monoclonal Antibody, Clone S388A-10. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin R Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin R Antibody. (D) Composite.
ANK1 / Ankyrin Antibody - Western Blot analysis of Rat Brain showing detection of ~200 kDa Ankyrin-R protein using Mouse Anti-Ankyrin-R Monoclonal Antibody, Clone S388A-10. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Ankyrin-R Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.
ANK1 / Ankyrin Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin R Monoclonal Antibody, Clone S388A-10. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin R Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin R Antibody. (D) Composite.
ANK1 / Ankyrin Antibody - Western Blot analysis of Rat Brain showing detection of ~200 kDa Ankyrin-R protein using Mouse Anti-Ankyrin-R Monoclonal Antibody, Clone S388A-10. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Ankyrin-R Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.
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Monoclonal Mouse anti‑Human ANK1 / Ankyrin Antibody (clone S388A‑10, Atto 390, IHC, IF, WB) LS‑C777147

Monoclonal Mouse anti‑Human ANK1 / Ankyrin Antibody (clone S388A‑10, Atto 390, IHC, IF, WB) LS‑C777147

Antibody:
ANK1 / Ankyrin Mouse anti-Human Monoclonal (Atto 390) (S388A-10) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Atto 390, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C777147-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ANK1 / Ankyrin Mouse anti-Human Monoclonal (Atto 390) (S388A-10) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Atto 390, Unmodified
Other formats:

Specifications

Description
Ankyrin antibody LS-C777147 is an Atto 390-conjugated mouse monoclonal antibody to Ankyrin (ANK1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human ANK1 / Ankyrin
Synonyms
ANK1 | ANK-1 | Ankyrin-1 | Ankyrin-R | Erythrocyte ankyrin | ANK | Ankyrin | Ankyrin 1, erythrocytic | SPH2 | SPH1
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
S388A-10
Conjugations
Atto 390. Also available conjugated with APC, Atto 488, Atto 594, Biotin, FITC, PerCP.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R
Specificity
Detects ~200 kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANK1 / Ankyrin
P16157 NM_020476 NP_065209.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024