Human ANK1 / Ankyrin

Goat

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-QIVKRASLKRGKQ, from the C-Terminus of protein sequence according to NP_000028.3NP_065208.2NP_065209.2NP_065210.2NP_065211.2NP_065213.2.

aa1971-1983

Human ANK1. This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).

• Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments gave approx. 19-23 kD bands in Human Skeletal Muscle lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kD bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kD according to NP_065211.2. The 19-22 kD bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16157 NCBI: NM_020476 NP_065209.2