Target
Human ANGPTL4
Synonyms
ANGPTL4 | Angiopoietin-like 4 | Angiopoietin-related protein 4 | Angiopoietin-like protein 4 | Fasting-induced adipose factor | NL2 | PGAR | Pp1158 | ARP4 | FIAF | HFARP
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide corresponding to aa 79-101 (ACQGTEGSTDLPLAPESRVDPEV) of human ANGPTL4
Specificity
Recognizes the coiled-coil domain of human ANGPTL4. Detects a band of ~18kDa by Western blot. Weakly cross-reacts with human ANGPTL6. Does not cross-react with other ANGPTL family proteins.
Applications
- Western blot (1:2000 - 1:5000)
- ELISA (1:2000 - 1:5000)
Usage
ELISA - Direct and indirect: 1:2000-1:5000. Western Blot - 1:2000-1:5000 using ECL. suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee